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Training In Various Areas Of Human Assisted Reproduction Techniques

Note:

Hands on Training in Andrology Lab Protocols

During this course program each participant receives one-on-one training to develop knowledge and skills in following areas:

  • Semen sample collection and delivery.
  • Semen analysis.
  • Sperm morphology assessment (WHO and Tygerberg strict criteria).
  • Fructose detection in seminal plasma (qualitative)
  • Viability testing (Eosin & Nigrosin stain).
  • 24 hrs survival test
  • Hypo-osmotic swelling test.
  • Leukocyte detection (Endtz Test/Peroxidase staining).
  • Media preparation
  • Consent forms & documentation

Sperm preparation protocols:

  • Preparation of ejaculated sperm for ART:
    - Removal of Seminal plasma
    - Double density gradient technique
    - Swim-up method
  • Concentration method (for low count samples)
  • Method of retrieval of sperm from retrograde ejaculate*
  • Sperm retrieval and preparation of testicular biopsy specimens (rat or mouse specimens will be used)*

Cryopreservation protocols:

  • Vitrification-Spermtozoa, egg, embryo.
  • Human spermatozoa freezing:
    - By vapour freezing method
    - By Planar technique
  • Isolation and cryopreservation of sperm from testicular biopsy specimens*
  • Safety guidelines for andrology laboratory.

* Demonstration

Mode of teaching:

  • Lectures and multimedia presentations.
  • Interactive discussions.

Each participant will receive:

  • Course manual.
  • Certificate of training

Duration of hands on training: 7- 21 days

Hands on training in Assisted reproduction techniques (IVF/ICSI)

This course provides comprehensive hands on training in the laboratory practices of IVF and ICSI. The syllabus covers the following areas:

Hands-on Laboratory Training:

Culture techniques

  • Preparation of culture dishes for IVF/ IVF-ICSI cases
  • Embryo culture
  • Blastocyst culture
  • Preparation of media for Hyaluronidase
  • Preparation of media for vitrification of embryos and blastocysts

IVF Techniques

  • Pipette pulling and Pipetting techniques
  • Ovum pickup from follicular fluid
  • Oocyte handling
  • Denudation of oocytes for ICSI
  • Morphological assessment of oocytes
  • IVF procedure
  • ICSI: Intracytoplasmic sperm injection
  • Assessment of oocyte fertilization
  • Embryo handling
  • Embryo grading
  • Embryo transfer (loading of embryos into embryo transfer catheter)

Advanced IVF techniques:

  • ICSI
  • TESA
  • PESA

Andrology Techniques

  • Semen Analysis
  • Sperm preparation for IUI/IVF/ICSI
  • Method of retrieval of sperm from retrograde ejaculate *
  • Testicular sperm retrieval
  • Consent forms & documentation

Duration of hands on training: 14-days

Mode of teaching:

  • Lectures
  • Multimedia presentations
  • Interactive discussions

Each participant will receive:

  • Certificate of training
    *Demonstration, mouse oocytes/sperm will be used.

Hands on training in cryopreservation techniques

During this course program each participant receives hands on training in the following cryopreservation techniques:
Human spermatozoa freezing:

  • By vapour freezing method
  • By Planar technique
  • Isolation and cryopreservation of sperm from testicular biopsy specimens*

Vitrification:

  • Embryos
  • Blastocysts
  • Preparation of vitrification media
  • Consent forms & documentation

* Demonstration
Mode of teaching:

  • Multimedia presentation
  • Interactive discussions

Each participant will receive:

  • Course manual
  • Certificate of training

Duration of hands on training: 4-days

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